VEGF Isolation from Platelet Lysate

, M.Sc. Thesis Sharif University of Technology Sanaei, Reza (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor)

Abstract

Platelet-rich plasma as a juvenile to the narrow repertoire of orthopedic medicine, has risen hope to attain a generic autologous regenerative formula for different conditions. Vast plethora of GFs, cytokines and chemokines has endowed PRP with excellent regenerative properties, however not all of them would favor different conditions. Vascular endothelial growth factor has been demonstrated to be involved in the progress of Osteoarthritis by amplifying the catabolic processes which deteriorate the extracellular matrix of cartilage. Though cumbersome, the result of eliminating VEGF from platelet lysate would be promising. VEGF isolation from platelet lysate requires selectivity. By employing...

Hydrogel Design and Development for Osteoarthritis Treatment

, M.Sc. Thesis Sharif University of Technology Mirshafeeyan, Mahshad (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mashayekhan, Shohreh (Supervisor)

Abstract

"osteoarthritis" is one of the most common joint diseases. This disease has a close relationship with factors such as aging, weight gain, genetics, mechanical pressures, and severe injuries. Although the mechanism of this disease is not fully understood, inflammation is one of the main causes of its exacerbation and progression. controlling inflammation can greatly help in controlling the disease. One of the common treatment methods for osteoarthritis is intra-articular injection of hyaluronic acid and triamcinolone as anti-inflammatory drug. Hyaluronic acid is the main component of synovial fluid, with its viscosupplementation property, prevents bones from rubbing against each other in the...

Impact of the Shape of the Loaded Particles on the Mechanical Properties of the Scaffolds and their Interaction with Macrophages

, M.Sc. Thesis Sharif University of Technology Tymuri, Fatemeh (Author) ; Mashayekhan, Shohreh (Supervisor) ; Safari, Hanieh (Supervisor)

Abstract

Osteoarthritis is a common musculoskeletal disorder and the most prevalent form of arthritis. To date, no comprehensive cure has been reported for osteoarthritis, and its clinical management remains challenging. The primary therapeutic goals in the early stages of osteoarthritis are to increase joint mobility and control disease progression by continuously releasing drugs and molecules modulating inflammatory responses and reducing pain. Smart drug delivery platforms are one of the strategies to overcome the challenges of injectable and oral medications. Additionally, drug carrier particles can have different therapeutic targets based on their size and shape. Different therapeutic...

Analysis of Lower-Limb Muscle Synergies in Patients with Knee Osteoarthritis Before and After Physiotherapy Intervention

, M.Sc. Thesis Sharif University of Technology Mohammadi, Mohammad Reza (Author) ; Arjmand, Navid (Supervisor)

Abstract

Knee osteoarthritis is one of the most common musculoskeletal disorders, characterized by the gradual deterioration of the joint, reduced range of motion, chronic pain, and a decline in patients’ quality of life. This condition disrupts the neuromuscular system, leading to decreased motor coordination of the lower-limb muscles. The present study was designed to investigate the effects of a physiotherapy intervention on lower-limb muscle function as well as to analyze changes in muscle synergies in patients with knee osteoarthritis. Fourteen patients with moderate knee osteoarthritis were compared with eleven age-matched healthy individuals before and after undergoing physiotherapy treatment....