Sharif Digital Repository

Novel high-throughput device and their applications as the microextraction systems for pesticide multiresidue determination is the centre of this research. 96 extracting unit based on solid phase microextraction and micro-Solid phase extraction were constructed and applied for high-throughput extraction.
In the first work, a high-throughput SPME on 96-well plate together with gas chromatography-mass spectrometry was developed for the determination of pesticide residues in cucumber samples. A home-made Teflon block consisting ninety-six holes was constructed to 96 SPME fibers being positioned in. SPME fibers consisted of stainless steel rods with a 1.0 cm polydimethylsiloxane tubing... 

Polydimethylsiloxane (PDMS) is one of the most important organosilicon elastomers. This polymer has a wide application such as medical engineering due to the unique characteristics, variety and ease of manufacture. Nowadays, the use of nanoparticles such as carbon nanotubes for improving the properties of elastomers is highly regarded owing to the fact that the improvement of properties such as mechanical properties broadens the applications of PDMS. Diamond nanoparticles has been the focus of numerous researchers in the past decade. In fact, nanodiamond has a good mechanical abrasion and thermal properties. In addition, the investigations proved that the nanoparticle is valuable in terms of... 

In this study, the pervaporation method was used for desulfurization of gasoline. Thiophene was used as a sulfur agent and n-heptane as a representative of hydrocarbon compounds in gasoline. The composite membranes of the active PDMS layer containing the UiO-66 metal-organic framework nanoparticles are based on porous membrane PVDF. The effect of adding PVP polymer on the porosity of the PVDF membrane has been investigated. Scanning electron microscopy (SEM), Fourier transform infrared spectrum (FTIR) and X-ray diffraction (XRD) have been used to characterize nanoparticles and synthesized membranes. The effect of adding nanoparticles on the membrane performance and its sowelling effect in... 

Titanium nano-oxide (TiO2) coatings have been used for self-cleaning, antimicrobial, and dust-proof designs. The degree of wetting of a solid surface with water in humid air depends on how the surface tension of the existing phases relates. The ratio between these tensions estimates the contact angle between a drop of water and the surface on which it is placed. A contact angle above 150 º is essential for surface overflow. In this study, 18 different combinations of titanium oxide and FAS, PDMS and PMS nanoparticles were obtained with a static contact angle of 165 to 150 º and a dynamic contact angle of 7 º.1 to 4.5 º. Following the measurement of water droplet slip speed on a sloping... 

In recent years, advances have been made in the field of miniaturization of the devices (in order to increase their efficiency, lessen the materials needed for the constructions and experiments, reduce costs and energy usage) resulting in the dramatic increase of surface to volume ratio in these devices. This progress has led to the dominance of surface forces which can be used to control very important interface phenomena having a special application such as electrokinetic. Using today’s manufacturing technology; it is possible to construct micro-nanochannels made from different materials such as silicon, glass, quartz, polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA) whose... 

Polymerase chain reaction, abbreviated as PCR, is a method of amplifying the number of copies of a desired DNA sequence through special protocols. The rapid advance of microfluidic devices and the emerging concept of digital microfluidics has resulted into the development of digital droplet PCR (ddPCR) systems with their significant uses in the detection of rare mutations, cancer diagnosis, and surveillance. A large number of micron-sized droplets are required to perform ddPCR. in this study, To investigate the gradient of confinements induced droplet self-breakup mechanism, we carried out the computational fluid dynamics (CFD) simulations for the two-phase flow using a commercial software... 

The aim of this research is to create coatings with antibacterial and superhydrophobic properties. To evaluate this, the sourness angle test, bacterial culture and halo diameter test have been used. The results show that 316L stainless steel containing PDMS+TiO2+7%Ag3PO4 coating, which is applied by immersion method on the substrate, has a water contact angle of 152 degrees, the number of bacteria cfu.cm2 is 44000 and the halo diameter is 24 mm. which has the best performance among other examples. In order to increase the adhesion of coatings on 316L stainless steel, first heat treatment was performed and then the samples were placed in H2SO4:H2O2 solution for 1 hour to form its OH- groups... 

Polymerase Chain Reaction (PCR) is a process in which a special piece of a gene is amplified millions of times over a short period. The advancement of gene amplification devices over the past years and attention to droplet-based methods have led to the development of droplet digital PCR. This method is very accurate in the diagnosis of diseases, so it can identify several different types of defective genes at the same time. In the present study, a microfluidic droplet-based chip was designed and fabricated for digital PCR amplification. The chip consists of microchannels for droplet formation as well as a reservoir to store droplets. Droplet formation was carried out by the flow-focusing...