Sharif Digital Repository

Today, almost all the country's needs in the field of industrial enzymes are met through imports. In this regard, much attention has been paid to the industrial production of various enzymes in the country. The production of recombinant proteins through strain engineering is one of the most important ways to meet this demand due to its many benefits. One of the most important microorganisms that is widely used in the enzyme industry is Bacillus subtilis. Due to its thick peptidoglycan wall, this gram-positive bacterium hardly accepts engineered carriers for genetic modification as hosts. The aim of this study was to evaluate the permeability of the wall and cell membrane of Bacillus subtilis... 

This research explains the structure of “belief transformation” from a philosophical perspective. We experience the whole world as it is, without realizing that it is formed by invisible networks of beliefs, linguistic structures and bodily habits. A network that, like a personal “paradigm”, determines what we see, how we think and how we feel. Using an interdisciplinary perspective, this thesis combines key concepts from phenomenology (Husserl, Heidegger), philosophy of language (Wittgenstein, Gadamer) and philosophy of science (Kuhn, Quine) to provide a model for understanding this fundamental transformation. The argument is that transformation is not the final accumulation of knowledge,...