Loading...

Synthesis of Amino Acids Schiff Base Complexes of Gadolinium and Study of Their Interaction with BSA

Abedi, Najmeh | 2008

802 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 39194 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Mohammadi Boghaei, Davar
  7. Abstract:
  8. Salicylaldehyde-amino acid Schiff base complexes are used as non-enzymatic models for the metal-pyridoxal (Vitamin B6) amino acid Schiff base systems which are key intermediates in many metabolic reactions of amino acids catalyzed by enzymes which require pyridoxal as a cofactor (transamination, decarboxylation, α and β elimination, racemization, etc.). In addition, complexes of amino acid Schiff bases are considered to constitute new kinds of potential anticancer, antitumor, antiradical and antibacterial reagents. On the other hand nuclear magnetic resonance imaging (MRI), has become a powerful tool for clinical diagnostics thanks to the development of a new class of pharmaceuticals administered to patients to enhance the contrast between normal and diseased tissues. Such contrast agents are mostly based on metal complexes of paramagnetic metal ions, since they enhance the relaxivity of water protons, the main factor determining the intensity of the MRI image. In this context, complexes of Gd(III) have received the greatest interest because of their high magnetic moments and relaxation efficiency. In this study,regarding to importance of the mentioned factor, several new amino acid Schiff base complexes of gadolinium are synthesized and characterized. One of these complexes is water soluble, so has biological importance. The drug-protein intraction may result in the formation of a stable protein-drug complex, which has important effect on the distribution, free concentration and the metabolism of drug in the blood stream. Afterwards, considering the importance of study of drug-protein interaction, the interacion of water soluble synthesized gadolinium complex with BSA, is investigated by fluorescence and absorbance spectroscopy. Study of effective interaction of synthesized model complex with BSA (regardind to its structural homology with HSA as the most important blood protein of the human), is important in development of bioinorganic chemistry and may cause this complex a suitable and valuable candidate in drug designing.
  9. Keywords:
  10. Spectrofluorometry ; Contrast Agents ; Magnetic Resonance Imagin (MRI) ; Bovine Serum Albumin (BSA) ; Schiff Base Complexes ; Amino Acid

 Digital Object List

 Bookmark

No TOC