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Empirical Study for Removal of Oil Contamination by Thermophilic Bacteria

Babaeivelni, Kamel | 2010

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 40660 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vossoughi, Manuchehr; Alemzade, Iran
  7. Abstract:
  8. Biodegradation has always been as a proper method for treatment of oil contamination. Accordingly, several investigations have been done by mesophilic bacteria for biodegradation of hydrocarbons while few studies have been conducted about thermophilic bacteria.
    There are many factors like high growth rate of thermophilic bacteria and good resistance of enzymes secreted from thermophiles against physical and chemical variations lead to the extensive use of thermophilic bacteria for removal of oil contamination. The important factors should be optimized in order to choose a suitable method for the removal of oil contaminations. Optimal conditions for oil contamination removal by thermophilic bacteria (BBRC9013) have been investigated in this project by statistical Response Surface methodology approach. In this regard, four important factors including temperature, mixing, pH and nitrogen source were studied and it was determined that the nitrogen source and temperature have the most impressive effect on the removal of oil contamination. Finally, Quadratic model was selected to predict the oil removal efficiency and the optimal values for temperature, mixing, pH and nitrogen source were 59.2 ᵒC, 151.15 rpm,6.57 ,NH4Cl, respectively. The best efficiency for the removal of oil contamination was 79.62% under optimum conditions. The experimental result was conducted in order to study whether the model can predict the oil removal efficiency to a good extent or not. The experimental value of removal efficiency is about %76.93 which is almost close to the value of model and this verified that mentioned model can predict oil removal efficiency appropriately.
  9. Keywords:
  10. Biodegradation ; Bacteria ; Oil Pollution ; Thermophilic Bacteria

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