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Molecularly Imprinted Polymers for the Treatment of Disease

Nematollahzadeh, Ali | 2010

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 41090 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abd Khodaei, Mohammad; Shojaei, Akbar
  7. Abstract:
  8. In the present investigation, experimental design, synthesis and evaluation of Verapamil, Albumin, Immonuglubline G and Trypsin imprinted polymers and finally Albumin imprinted poly(dopamine) nano-layer were presented. A bimodal polymerization approach as a combination of precipitation and dispersion polymerization was taken towards producing 500 nm particles for verapamil imprinting purpose. In this case the imprinting factor was 2.17. In a challenge for producing spherical particles with predefined size and pore properties the hierarchical polymerization in packed beds, using porous silica particles (particle size ca. 40 µm) was developed, as well. The method resolving the problems entangled in protein imprinting, such as protein incomaptiblity with polymerization solution, low capacity and high mass transfer resistance, exhibited adsorption of 140 mg HSA and 100 mg IgG per gram of dry polymer. The HSA and IgG imprinted polymers adsorbed the corresponding templates in 25 and 120 min, repectively. For the fabrication of robust protein imprinted polymers for on-line protein separation, an ultrathin layer (3-12 nm) of polydopamine on porous silica particles was imprinted with human serum albumin and the capability of the imprinted polymers in separation of Albumin from albumin solution or human serum in HPLC columns was evaluated. The capability of such column in protein separation was the main specificity of such polymers
  9. Keywords:
  10. Molecularly Imprinted Polymers ; Small and Large Template Molecule ; Hierarchichal Polymerization ; Protein Separation

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