Spectrophotometric Determination of D - L-Penicillamine Using Red Shift on the Surface Plasmon Resonance Band of Au Nanoparticles

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Azargun, Mohammad | 2011

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 41726 (03)
University: Sharif University of Technology
Department: Chemistry
Advisor(s): Hormozi Nezhad, Mohammad Reza
Abstract:
We report herein the development of a highly sensitive colorimetric method for detection of D- Penicillamine using citrate capped gold nano particles (Au-NPs) .This assay relies upon the distance-dependent of gold nanoparticles surface plasmonic resonance band of gold nanoparticles. By replacing the thiol-containg chelator drug, D-Penicillamine, with citrate on the gold nano particles surface, a new peak appearing at a longer wavelength intensifies and shifts further to the red from the original peak position which depends on ionic strength, D- Penicillamine and gold nanoparticles. The ratio intensities of absorbance at longer wavelength (630 nm) to original wavelength (521 nm) allow the simple quantitative spectro photometric detection of D-Penicillamine in pharmaceutical and biological samples. The calibration curve drived from the ratio intensities of absorbance at 630 nm to 521 nm, and it was linear in the concentration range of 5 × 10-6 – 300 × 10-6 M. The detection limit (3σ) for D-Penicillamine was 3× 10-6
Keywords:
Gold Nanoparticle ; D-Penicillamine ; Nanoparticles Aggregation ; Plasmonic Red Shift ; Colorimetric

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