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Electrophoretic Deposition of Alginate-Bioglass-Nanodiamond Nanocomposites and Evolution of their Bioactivity

Mansoorianfar, Mojtaba | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42131 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Simchi, Abdollreza
  7. Abstract:
  8. Recently, diamond nanoparticles have attracted interest for biomedical applications such as drug delivery, targeted cancer therapies, fabrication of tissue scaffolds and biosensors. In the present work, elecrophoretic deposition (EPD) of nanodiamond-bioactive glass-alginate nanocomposite was studied. In vitro bioactivity and biocompatibility of the nanocomposite were evaluated in simulated body fluid (SBF) and by MTT assay. The EPD process was performed under different conditions in order to obtain a uniform coating on the surface of 316L stainless steel substrate. The stability of the suspension was determined via optical sedimentation method and zeta potential analysis. It was found that at a concentration of 0.7 gr/lit nanodiamond, 1.3 gr/lit Bioglass and 0.6 gr/lit alginate a uniform film is obtained after 3 min EPD at a field strength of 10 V/cm. Characterization of the coating by scanning electron microscopy (SEM) and simultaneous thermal analysis (STA) showed that nanodiamond and bioactive glass particles were homogeneously distributed throughout the polymer matrix. The amount of particles in the matrix polymer was determined to be 50 wt%. The results of in vitro bioactivity test in Kokubo's SBF medium up to 4 week showed that calcium phosphate (apatite) particles are formed on the surface of the film, exhibiting its bioactivity. Although the presence of diamond nanoparticles decreased the EPD rate of the nanocomposite suspension, no influence on the bioactivity was noticed. Meanwhile, MTT assay by using MG63 osteoblast cells revealed that the presence of nanodiamond particles in the alginate-bioactive glass coating increases the cell viability
  9. Keywords:
  10. Alginate ; MTT Assay ; Bioactive Glass ; Diamond Nanoparticle ; Bioglass

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