Supercritical Carbon Dioxide Extraction of Catechins from Green Tea Leaves

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Hassan Kashi, Sima | 2010

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 41459 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Goodarznia, Iraj
Abstract:
Green tea has been receiving significant attention in recent years due to its high content of catechins; Epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC) and especially epigallocatechin gallate (EGCG). As powerful antioxidants, these compounds are believed to have several health benefits. In this study, we used supercritical CO2 with ethanol as co-solvent to extract catechin derivatives from green tea and we investigated the effects of experiment parameters on the extraction. Experiments, designed according to Taguchi algorithm, were conducted with green tea particles of four average diameters of 1500, 855, 605 and 427.5 µm, in an interval of 35- 55 ̊C for temperature and 100-160 bar for pressure. Samples were analyzed by high performance liquid chromatography. Analysis of Variance (ANOVA) on experimental data showed that temperature is the dominant factor in EGCG and ECG extraction, and for EC and caffeine, particle diameter has the highest effect, and as it is expected, smaller particles give higher extracted quantities
Keywords:
Catechin ; Extraction ; Supercritical Fluid ; Carbon Dioxide ; Green Tea

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