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Spectrophotometric Determination of Arginine by Aggregation of Spherical Gold Nanoparticles

Pezeshkian, Zahra | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 43257 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Hormozinezhad, Mohammad Reza
  7. Abstract:
  8. Spectrophotometric determination of arginine by aggregation of spherical Au nanoparticles Arginine has been classified as a ‘semi-essential’ or ‘conditionally-essential’ amino acid. This amino acid plays a crucial role in many biological path ways. Hence, its determination in biological body fluids such as blood plasma and urine is important for clinical diagnosis of various diseases. Herein we report the development of a sensitive colorimetric method for the determination of arginine based on aggregation of the citrate capped gold nanoparticles (Au NPs). Reaction contains replacement of citrate ions with guanidinium groups of arginine followed by establishing a chemical bond between amino acid groups and carboxylic acid groups of arginine. As a result of aggregation, which could be affected by the ionic strength, pH and concentration of Au NPs, the plasmon band at around 520nm decreases gradually along with formation of a new red shifted band. The calibration curves, which are derived from the intensity ratios of the absorbance at-700 nm to original wavelength of 520 nm, display two linear relations with arginine concentration. The first range is from 0.08 mM to 0.2 mM and the second is from 0.2 mM to 2 mM. The calculated detection limit in this method is 0.02 mM.
  9. Keywords:
  10. Gold Nanoparticle ; Nanoparticles Aggregation ; Colorimetric ; Plasmonic Red Shift ; Arginine ; Colorimetric Determination

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