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Experimental Study and Process Design of Oil Extraction from Microalgae Chlorella Vulgaris to Produce Biodiesel

Hassani, Iman | 2012

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 43976 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Farhadi, Fathollah; Vossoughi, Manoochehr
  7. Abstract:
  8. The experimental investigation of biodiesel production stages from microalgae especially lipid extraction stage was conducted in this thesis. The optimal rate of biomass productivity and optimal method in lipid extraction for biodiesel production was determined.
    A photobioreactor was designed and constructed to cultivate microalgae C. Vulgaris according to researchers’ works and obtained results in this study. The process parameters were examined and the optimal parameters were obtained according to the necessary tests which were carried out. Then, several methods of lipid extraction using different solvents such as chloroform, methanol, and n-hexane were investigated and the best combination of solvents was proposed for lipid extraction.
    According to the results, the optimal condition for growing of microalgae was obtained with aeration of 6 lit/min with 3% CO2, light regime of 16 hours light and 8 hours dark, and CO2 injection regime of 45 sec. injection and 3 min. of stoppage. A rapid growth of microalgae and productivity rates of 0.11 g L-1 day-1 was obtained in this condition.
    The Folch method in lipid extraction of freeze-dried biomass with yield of 31.6% has the best extraction performance among the extraction methods. Main fatty acid methyl esters composition was represented as the following: C14:0 (22.95 %), C16:1 (41.13%), C18:2 (14.49%), C18:3 (5.65%). Biodiesel production rate of 18.37 mg L-1 day-1 was obtained
  9. Keywords:
  10. Chlorelavulgaris ; Transesterification Reaction ; Biodiesel ; Oil Extraction ; Microalgae

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