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Optimization and Offering a kinetic Model for Enzymatic Hydrolysis of Cellulosic Materials in a Membrane Bio-reactor

Kashefi, Saeed | 2013

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 44637 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Soltanieh, Mohammad; Shaygan Salek, Jalaloddin
  7. Abstract:
  8. Regarding decreasing resources of fossil fuels and their increasing costs in addition to their environmental pollutions, today, producing bio fuels from wastes such as lignocellulosic materials is of great interest. A fundamental stage in conversion of lignocellulosic materials into bio fuels, such as bio ethanol, is the enzymatic hydrolysis of these materials to produce reducing sugars. Researches have shown high costs of this stage. Optimization of enzymatic hydrolysisconditions is a major step in economization of this process.In this work corn hull is used to produce glucose. Corn hull is a kind of lignocellulosic material that due to high carbohydrate content is used as substrate. Our goal is to achieve an optimized condition, such as salt concentration, surfactant concentration and stirrer speed, in which glucose production from corn hull would be maximum and product inhibitionreduced as much as possible. Enzymatic hydrolysis reaction takes place in a membrane bio reactor that continuously removes the products from reaction environment. Experimental design and optimization of operation conditions was done by “Design Expert 7.0” software. A quadratic model is presented for predicting glucose production based on process parameters. After achieving the optimized condition (NaCl concentration= 42.08 mM, surfactant concentration= 0.01vol% and impeller speed=607 rpm), an experiment was done to evaluate the predicted results by the model. Results (a relative 5% error) reveal the validity of the model. A 1.522×10-5 (L/g.h)value of enzyme deactivation rate constant shows that enzyme activity was constant through the reaction and continuous product removal is so efficient. After optimization of operation policy, kinetic parameters of the enzymatic hydrolysis reaction were derived in the optimized condition.According to the results a continuous products removal from the reaction environment has a major role in reducing product inhibition. The consequence is a reduced consumption of enzymes and decreasing operational costs of the process
  9. Keywords:
  10. Corn Fiber ; Inhibition Effect ; Membrane Bioreactor ; Operational Optimization ; Continuous Product Removal

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