Evaluation of Process Partitioning of Enzyme in Polymer-Salt Aqueous Two-PHase Systems

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Kamankesh, Amin | 2013

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 45698 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Alamzadeh, Iran; Vossoughi, Manouchehr; Pazuki, Gholamreza
Abstract:
In this research, the amount of partitioning Invertase to be evaluated in nine systems, these systems contain Peg 2000,Peg 4000 & Peg 6000 with three different salt.In first, Binodal curve for nine systems to be drown by cloudy point method, then five point be selected in each system such as sample feed. Invertase in every systems to be solved in constant weight fraction. The analysis of every phasesto be achieved by Merchuk method. For correlation data of this method to be used by Othmer-Tobias method and then using equation Mrchvk ensure the accuracy of phase analysis , analysis of the results obtained with the UV method was studied.Accuracy of UV analysis was studied by using lattice theory of Flory−Huggins.Important parameters on partitioning coefficient of Invertase such as the effect of molecular weight and different salts in each system were evaluated.The DNS test using a sample rate of decline of activity in both the upper and lower phase wasstudied
Keywords:
Aqueous Two-Phase System ; Modeling ; Invertase Enzyme ; Partitioning Coefficient

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