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Spectrophotometric Determination of Copper Ions and Pamidronate Disodium Based on Changing the Surface Plasmon Resonance Band of Au Nanoparticles

Abbasi-Moayed, Samira | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 46131 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Hormozi-Nezhad, Mohammad Reza
  7. Abstract:
  8. In recent years, metallic nanoparticles, specially gold and silver nanoparticles, have received great attention for biological and metal ions analysis, due to the surface plasmon resonance phenomena. Gold nanoparticles (AuNPs) have recently gained much interest in colorimetric probe based on aggregation or anti-aggregation of AuNPs, due to their simplicity and having high order of magnitude extinction coefficients, in addition to the dependence of surface plasmon resonance wavelength and intensity on the interparticles distances. In the first part of this thesis, a highly sensitive colorimetric method for detection of copper ions based on anti-aggregation of citrate capped AuNPs was developed. In the presence of copper ions, D-Penicillamine prefers to coordinate to copper rather than acting as an aggregation agent for AuNPs; thus Cu2+ inhibits the aggregation of AuNPs. Under the optimum experimental conditions, a linear calibration curve for Cu2+ was obtained within range of 5.00×10-8-1.85×10-6 M. Detection limit (3σ) of 3.00×10-8 M was obtained for this method. The method was applied successfully to the determination of copper ions in tap water, plour cascade water and mineral water.
    In the second part of the research, we have developed a simple colorimetric assay based on aggregation of AuNPs for the determination of Pamidronate disodium, which is a bisphosphonate drug. This drug exhibits affinity toward the surface of gold nanoparticles and causes the aggregation of AuNPs by hydrogen bonding interactions. The interparticle interactions lead to a decrease in the plasmon band around 520 nm and the formation of a new red shifted band. The calibration curve for Pamidronate is derived from the ratio of the absorption intensity at 650nm respectively to the original wavelength (520 nm) which was linear in the concentration range of 5.0×10-5-22.5×10-5 M. The detection limit (3σ) for Pamidronate was found to be 4.3×10-5 M. This method was applied to the determination of Pamidronate disodium in blood plasma sample
  9. Keywords:
  10. Gold Nanoparticle ; Colorimetric ; Anti-Windup ; Cupper (II) Ion ; Aggregation Treatment ; Pamidronate Disodium

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