Dosign and Construction of Natural Polymers-based Hydrogels for Cartilage Tissue Engineering

Nasiri, Bita | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 46793 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Hajebrahimi, Zahra
  7. Abstract:
  8. Once damaged, articular cartilage has very little capacity for spontaneous healing because of the avascular nature of the tissue. Although many repair techniques have been proposed over the past decades, none has successfully regenerated long-lasting tissue to replace damaged cartilage. Tissue engineering have recently demonstrated tremendous approaches for regeneration of cartilage tissue lesions. Tissue engineering is based on three principles: cells, scaffolds for cell adhesion and growth factors. Three-dimensional biodegradable scaffolds play an important role in tissue engineering. In this study, novel cross-linked hybrid chitosan/ECM scaffolds were prepared for articular cartilage regeneration. The influence of chitosan amount on structural and mechanical properties of three scaffolds with different concentration of chitosan 4%, 5% and 6% was evaluated. This investigation were also applied on two scaffolds with 1% and 2% ECM simultaneously to study the effects of ECM concentration variations. The results demonstrated that the scaffold with 5% chitosan showed best mechanical and structural properties. The insignificant impact of ECM concentration on mechanical and structural scaffold’s properties is also proven. In the next step, for showing the effect of ECM presence on scaffold’s biocompatibility and biodegradability, three scaffolds with 1%, 2% and 3% ECM were constructed. By considering the conclusions which are taken from the result of SEM pictures, biodegradability tests, MTT assay and swelling ratio, the scaffold which was consisting of 5% chitosan and 2% ECM was shown to have accepted chondrocyte viability. Our results suggest that the hybrid novel scaffold of chitosan/ECM could have potential use in cartilage tissue engineering
  9. Keywords:
  10. Chitosan ; Decellularization ; Extracellular Matrix ; Cartilage Tissue Engineering ; Hybrid Scaffold

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