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Spectrofluorimetric Determination of Copper Ions Based on Fluorescence Resonance Energy Transfer and Spectrofluorimetric Determination of Iron (III) Ions in the Prescence of Iron (II) Ions Based on Fluorescence Quenching

Taghipour, Mitra | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 47293 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Hormozi Nezhad, Mohammad Reza
  7. Abstract:
  8. The use of fluorophores in metallic and non-metallic analysis, have received great attention for chemical sensing due to the high sensitivity and selectivity. Fluorophores through participating in emission mechanisms, interact with analyte and the emission intensity changes, which leads to determination of analyte concentration. These mechanisms are included fluorescence resonance energy transfer and fluorescence emission quenching. In the first part of this thesis, a highly sensitive probe for detection of copper ions was developed based on fluorescence resonance energy transfer due to the plasmonic peak of citrate capped gold nanoparticles and emission peak of FITC. Quenched emission of probe due to the adsorption of FITC on the AuNPs surface through isothiocyanate, was turned on because of the replacement of D-PC on the AuNPs surface through thiol group. In the presence of copper ions, D-Penicillamine prefers to coordinate to copper rather than replacing on the AuNPs surface; thus Cu2+ inhibits the increase of fluorescence emission. Under the optimum experimental conditions, a linear calibration curve for Cu2+ was obtained within range of 1-40 nM. Detection limit (3σ) of 0.33 nM was obtained for this method. The method was applied successfully to the determination of copper ions in mine water and food samples. In the second part of the research, we have developed a simple assay based on fluorescence emission quenching for the determination of iron (III). Due to the specific coordination interactions of iron (III) ion and hydroxyphenols on carbon dots surface, the fluorescence emission of the probe quenches. The calibration curve for iron (III) was linear in the concentration range of 1-500 µM. The detection limit (3σ) was found to be 0.56 µM. This method was applied to the determination of iron (III) in river water and spinach
  9. Keywords:
  10. Gold Nanoparticle ; Fluorescence Resonance Energy Transfer ; Cupper (II) Ion ; Fluorescence Isothiocyanate ; Fluorescence Quenching ; Ferrous Ion

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