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Designing, Computational Modeling and Fabrication of a Magnetic Centrifugal Microfluidic to Separate Circulating Tumor Cells from Blood Sample

Selahi, Amirali | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 47879 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir
  7. Abstract:
  8. Separating cells from a mixed sample is a required task in biotechnology and modern medicine, for example to isolate CTC’s that are of interest for doing therapy and diagnosing or doing research. A minute percentage of target cells must be separated from a large amount of unwanted cells. These target cells like CTC’s could be as rare as 1 target cell per millions of unwanted background cells. Hence Microfluidic cell sorting schemes based upon fluorescent labelling, electrophoresis, dielectrophoresis and magnetophoresis have been devised. Microfluidic magnetically-activated cell sorting (MACS) does not need any optical instrument or current source and electrode. By using an electromagnet or permanent magnet attraction of magnetically-labelled cells in a microfluidic channel is possible. These devices possess remarkable advantages in minimizing the time and cost associated with a routine biological analysis while improving reproducibility. Here a centrifugal magnetic microfluidic has been designed to separate rare circulating tumor cells (CTCs) from background blood flow. Besides novel small geometry which decreases the need of sample, an innovative method has been used that consists of two stages of separation, first separation due to magnetic field and second separation due to coriolis force caused by curved channel on a rotating disk. Combining these two stages together increases the purity of the desired cells and decreases the cost and difficulties of fabrication by eliminating the need for focusing channel and other very small mechanisms. Then separation of magnetic beads is added to the previous work. Finally this novel microfluidics has been fabricated and tested in laboratory
  9. Keywords:
  10. Magnetic Fields ; Cancer Cells ; Microfluidic System ; Cell Separation ; Circulating Tumor Cells (CTC) ; Magnetic Beads

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