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In vitro study of bare and poly (ethylene glycol)-co-fumarate coated superparamagnetic iron oxide nanoparticles for reducing potential risks to humans and the environment

Mahmoudi, M ; Sharif University of Technology | 2011

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  1. Type of Document: Article
  2. Publisher: Nova Science Publishers, Inc , 2011
  3. Abstract:
  4. For risk free application of nanoparticles in life science, energy and the environment, it is essential to understand their biological fate and potential toxicity. The application of iron oxide nanoparticles for drug delivery has been one of the most promising researches in the field of nanotechnology. However, there are two major problems associated with magnetically targeted deliveries that still need close attention: • As the drug is coated on to the particle surfaces, there is the possibility of faster drug release (Burst Effect). Therefore, after reaching to the specific site, there is very low amount of drug for delivery. To overcome this problem, some researchers have used conjugation drug-polymer systems. However, these systems need special procedures for breaking bonds between the coating polymer and the drug at targeted sites. • Once the surface-derivatized particles are inside cells, it is probable that cells may digest the polymeric coatings, leaving the bare particles exposed to other components and organelles within the cytoplasm, which could influence the overall integrity of the cells. To overcome these problems, in the present work we examine the use of poly (ethylene glycol)-co-fumarate (PEGF) as a molecular coating for iron oxide nanoparticles. The coating has been cross-linked and rigid shells are formed on the surface of nanoparticles. The combination of superparamagnetic nanoparticles with PEGF shells (e.g. hydro-gel) and further trapping of drug in hydro-gel substrate (e.g. cross-linked PEGF) showed high potential of decreasing the burst effect as well as offering good biocompatibility. As a result, the nanoparticles can be used reliably for both transport and imaging applications. In addition, digestion of the polymer coating can be delayed inside the cells. The extent of biocompatibility has been traditionally evaluated by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (MTT assay) methods. Our results suggest that compositions based on unsaturated aliphatic polyesters are potentially more useful for developing novel carriers for drug and gene delivery applications
  5. Keywords:
  6. Biocompatibility ; Magnetic nanoparticles ; MTT assay ; Poly (ethylene glycol)-co-fumarate
  7. Source: Handbook of Sustainable Energy ; May , 2011 , Pages 649-666 ; 9781608762637 (ISBN)
  8. URL: http://www.ncbi.nlm.nih.gov/pubmed/19433870