Experimental Studies for Lactase Immobilization on the Polymer base and Characterising

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Derakhshan, Elahe | 2015

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 48091 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Yaghmaei, Soheyla
Abstract:
Immobilization of β-D-galactosidase represents an important approach for development of lactose hydrolysis technology.Chitosan proved to be an excellent carrier for β-Dgalactosidase immobilization, by both covalent binding and sol-gel entrapment combined with adsorption. Among different preparation methods of chitosan support particles for covalent binding, emulsion cross-linking was the most efficient, producing narrow-sized microspheres, without aggregation.The best process parameters of β-D-galactosidase immobilization by covalent coupling on chitosan microspheres were found as 3% glutaraldehyde cross-linker concentration, enzyme loading of 27 mg protein/g dry support, 6 h reaction time, and reduction of the formed covalent double bonds to more flexible secondary amino bonds. In the best conditions, 23.5% of the total enzymatic activity was recovered after immobilization
Keywords:
Lactase Enzyme ; Enzyme Stabilization ; Nanoparticles ; Chitosan Nanoparticles ; Microparticles ; Beta Galactosidase Enzyme

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