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Experimental Study of Partitioning of Lysozyme Enzyme in Aqueous Two-Phase Systems based on Surfactants

Barjasteh, Mahdi | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 48368 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Bastani, Dariush; Vosoughi, Manochehr
  7. Abstract:
  8. In this study, aqueous two-phase systems (ATPS) containing Tween 20 as a biocompatible nonionic surfactant and three different salts (NH4HPO4, NH4CH3COO and Mg(CH3COO)2 ) were prepared and their extractive potential for Lysozyme enzyme was evaluated. These systems have surfactant-rich top phase and salt-rich bottom phase. Phase equilibrium diagrams at 23 ° C were obtained by using the cloud point method and the results were successfully fitted to the Merchuk correlation and their trends were correlated with the effective excluded volume (EEV) parameter. Afterward, the partitioning behavior of the lysozyme and its extraction efficiency in the systems of Tween 20/diammonium hydrogen phosphate, Tween 20/ magnesium acetate, Tween 20/ammonium acetate at 23 ° C was determined and the effect of salt type, tie line length and the addition of silica nanoparticles were studied. It was observed that nanoparticles dramatically increase the partition coefficients of enzyme. According to the experimental data, diammonium hydrogen phosphate salt system was suitable for the extraction of lysozyme in the down phase with extraction efficiency of 92% and ammonium acetate salt system was suitable for the extraction of lysozyme in the top phase with extraction efficiency of 82%
  9. Keywords:
  10. Aqueous Two-Phase System ; Surfactants ; Lysozyime Protein ; Partitioning ; Tween 20 Surfactant

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