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Biodesulfurization of dibenzothiophene by a newly isolated Rhodococcus erythropolis strain

Davoodi Dehaghani, F ; Sharif University of Technology | 2010

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  1. Type of Document: Article
  2. DOI: 10.1016/j.biortech.2009.08.058
  3. Publisher: 2010
  4. Abstract:
  5. A new dibenzothiophene (DBT) desulfurizing bacterium was isolated from oil-contaminated soils in Iran. HPLC analysis and PCR-based detection of the presence of the DBT desulfurization genes (dszA, dszB and dszC) indicate that this strain converts DBT to 2-hydroxybiphenyl (2-HBP) via the 4S pathway. The strain, identified as Rhodococcus erythropolis SHT87, can utilize DBT, dibenzothiophene sulfone, thiophene, 2-methylthiophene and dimethylsulfoxide as a sole sulfur source for growth at 30 °C. The maximum specific desulfurization activity of strain SHT87 resting cells in aqueous and biphasic organic-aqueous systems at 30 °C was determined to be 0.36 and 0.47 μmol 2-HBP min-1 (g dry cell)-1, respectively. Three mM DBT was completely metabolized by SHT87 resting cells in the aqueous and biphasic systems within 10 h. The rate and the extent of the desulfurization reaction by strain SHT87 suggest that this strain can be used for the biodesulfurization of diesel oils
  6. Keywords:
  7. Dibenzothiophene ; 2-hydroxybiphenyl ; 2-Hydroxybiphenyl (2-HBP) ; Biodesulfurization ; Biphasic ; Biphasic systems ; Desulfurization reaction ; Dibenzothiophenes ; Diesel oil ; Dry cells ; HPLC analysis ; Methylthiophene ; Oil contaminated soil ; Organic-aqueous system ; PCR-based detection ; Resting cells ; Sulfur source ; Diesel fuels ; Soil pollution ; Sulfur ; Thiophene ; Desulfurization ; Dibenzothiophene derivative ; Bacterium ; Cell organelle ; Diesel ; Aqueous solution ; Bacterial growth ; Bacterial metabolism ; Bacterium isolate ; High performance liquid chromatography ; Microbial activity ; Nonhuman ; Polymerase chain reaction ; Priority journal ; Rhodococcus erythropolis ; Biodegradation, Environmental ; Biotechnology ; Chromatography, High Pressure Liquid ; Dimethyl Sulfoxide ; DNA, Bacterial ; DNA, Ribosomal ; Polymerase Chain Reaction ; Rhodococcus ; Soil Microbiology ; Soil Pollutants ; Sulfones ; Temperature ; Thiophenes ; Asia ; Eurasia ; Middle East ; Bacteria (microorganisms)
  8. Source: Bioresource Technology ; Volume 101, Issue 3 , 2010 , Pages 1102-1105 ; 09608524 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0960852409010335