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  1. طراحی و ساخت داربست نانوفیبری زیست فعال به منظور بکارگیری در مهندسی بافت سیستم عصب مرکزی
طراحی و ساخت داربست نانوفیبری زیست فعال به منظور بکارگیری در مهندسی بافت سیستم عصب مرکزی
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طراحی و ساخت داربست نانوفیبری زیست فعال به منظور بکارگیری در مهندسی بافت سیستم عصب مرکزی

راستی بروجنی، فاطمه Rasti Boroojeni, Fatemeh

Design and Fabrication of Bioactive Nano Fibrous Scaffold for Central Nervous Tissue Engineering

Rasti Boroojeni, Fatemeh | 2016

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 48982 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Abbaszadeh, Hojjatollah; Hasannejad, Zahra
  7. Abstract:
  8. A system of electrospun scaffold containing drug-loaded chitosan nanoparticles was introduced for using in spinal cord tissue engineering. In spinal cord injury treatment, the formation of glial scar after injury which contains proteoglycans and activate glial cells should be avoided. In this study, the release of Dexamethasone sodium phosphate (DEXP) as a steroid anti-inflammatory agent from electrospun nanofiber composite containing chitosan nanoparticles was investigated. DEXP is believed to act through its glucocorticoid receptors found in most neurons and glial cells. These receptors’ pathways are involved in the inhibition of astrocyte proliferation and microglial activation. Additionally, as an anti-inflammatory substance, DEXP reduces numerous pro-inflammatory cytokines. Bovine serum albumin (BSA) was used to improve the encapsulation efficiency and to overcome initial burst release of water soluble drug. DEXP-BSA-loaded chitosan nanoparticles were fabricated using ionic gelation method based on the interaction between positive charge of amino groups of chitosan and negative charge of sodium tripolyphosphate (TPP). The encapsulation efficiency of DEXP in chitosan nanoparticles without BSA was determined 30%, while the use of BSA in combination with DEXP increased encapsulation efficiency up to 77%. As electrospun nanofibers show great potential to mimic axon structure and can induce axonal regeneration, in the present study, suspension electrospinning was employed to fabricate poly-ε-caprolacton (PCL) containing DEXP-loaded chitosan nanoparticles with a core-shell structure. To obtain the desired scaffold electrical properties with controlled release of DEXP, a hybrid electrospun scaffold was fabricated using PCL containing DEXP-loaded chitosan nanoparticles and gelatin containing polyaniline/graphene (PAG) nanoparticles. The use of PAG nanoparticles improved electrical conductivity of the scaffold. The comparison between DEXP release profile from PCL/gelatin scaffold with and without chitosan nanoparticle revealed the key role of nanocarier to control the drug release
  9. Keywords:
  10. Bovine Serum Albumin (BSA) ; Electrospinning ; Nanofiber ; Nanoparticles ; Nerve Tissue Engineering ; Controlled Release ; Spinal Cord Injury ; Dexamethasone

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