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Designing and Improving Manganese Oxide Nanoparticles as a MRI Contrast Agent

Omid, Hamed | 2016

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 49517 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Maddah Hosseini, Hamid Reza
  7. Abstract:
  8. In this thesis, manganese oxide nanoparticles with about 5 nm size were synthesized via solvothermal to maximize surface and active sites for proton relaxation. The synthesized nanoparticles were coated with gentisic acid to acquire suitable colloidal stability and biocompatibility. The nanoparticles showed low cytotoxicity in MTT assay. Electrosteric mechanisim was employed for colloidal stability. This mechanisim doesn’t need high surface charge and thick coat which are vital for particles functionality. Then by cell culture eliminating and adding protamine sulfade, nanoparticles uptake were increased tremendously. In this thesis for first time Prussian blue was utilized for staining manganese in cells. Finally, relaxation rate of nanoparticles in MRI was studied, and 8.75 mM-1s-1 which is notable amount was measured for the synthesized nanoparticles. This relaxation rate is about twice of available gadolinium based drug. With creating mixed structure of MnO/Mn3O4 nanoparticles obtained dual mode contrast enhancement ability. It means with one injection obtaining both positive and negative contrasts would be possible. This is a new diagnostic potential
  9. Keywords:
  10. Manganese Oxide Nanoparticles ; Cytoxicity ; Magnetic Resonance Imagin (MRI)Contrast Agent ; Cell Uptake ; Cell Staining ; Relaxation Rate

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