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Surface Modification of Bacterial Cellulose-Reinforced Keratin Nanofibers using Pluronic/Gum Tragacanth Hydrogel Nanoparticles Produced by Concurrent gel Electrospray/Polymer Electrospinning Method
Azarniya, Amir | 2017
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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 49760 (07)
- University: Sharif University of Technology
- Department: Materials Science and Engineering
- Advisor(s): Simchi, Abdolreza; Tamjid, Elnaz
- Abstract:
- In this work, wool keratin/polyethylene oxide (PEO) nanofibrous scaffolds were fabricated by electrospinning method. Bacterial cellulose nanofibrils (BCNFs) were embedded in the electrospun keratin/PEO nanofibers. Incorporation of BCNFs into the nanofibers enhances their hydrophilicity, mechanical properties and cell viability, adhesion and proliferation. Water contact angle of the nanofibers decreased from 126˚ to 83˚by addition of 1 wt % BCNFs. A thermogelling hydrogel based on carboxylated pluronic (Pl-COOH) and gum tragacanth (GT) was fabricated and polymer conjugation was confirmed by FTIR and H-NMR spectroscopy. Morphological and viscoelastic properties of GT-grafted Pl-COOH hydrogels were evaluated under physiological conditions. Hydrogel particles were electrosprayed concurrently with electrospinning of keratin/PEO/BCNFs nanofibers and hydrophilicity, mechanical and biological characteristics were improved. Elastic modulus, tensile strength and elongation of the hydrogel modified nanofibers were increased by 31, 35 and 23 %, respectively. Hydrophilicity, cell viability, adhesion and proliferation of the hydrogel codeposited mats were promoted. The prepared hydrogel modified nanofibrous scaffolds are potential candidates for biomedical applications such as temporary skin grafts and skin tissue engineering
- Keywords:
- Bacterial Cellulose ; Electrospinning ; Hydrogel ; Electrospun Nanofibers ; Skin Tissue Engineering ; Pluronic ; Keratin ; Gum Tragacanth
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