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Multivariate optimization of hydrodistillation-headspace solvent microextraction of thymol and carvacrol from Thymus transcaspicus

Kiyanpour, V ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. DOI: 10.1016/j.talanta.2009.04.068
  3. Publisher: 2009
  4. Abstract:
  5. In this paper multivariate response surface methodology (RSM) has been used for the optimization of hydrodistillation-headspace solvent microextraction (HD-HSME) of thymol and carvacrol in Thymus transcaspicus. Quantitative determination of compounds of interest was performed simultaneously using gas chromatography coupled with flame ionization detector (GC-FID). Parameters affecting the extraction efficiency were assessed and the optimized values were 5 min, 2 μL and 3 min for the extraction time, micro-drop volume and cooling time after extraction, respectively. The amounts of analyte extracted increased with plant weight. The calibration curves were linear in the ranges of 6.25-81.25 and 1.25-87.50 mg L-1 for thymol and carvacrol, respectively. Limit of detection (LOD) for thymol and carvacrol was 1.87 and 0.23 mg L-1, respectively. Within-day and between-day precisions for both analytes were calculated in three different concentrations and recoveries obtained were in the range of 89-101% and 95-116% for thymol and carvacrol, respectively. © 2009 Elsevier B.V. All rights reserved
  6. Keywords:
  7. Carvacrol ; Headspace solvent microextraction ; Hydrodistillation ; Hydrodistillation ; Response surface methodology ; Chromatographic analysis ; Gas chromatography ; Ionization of gases ; Optimization ; Phenols ; Solvent extraction ; Solvents ; Surface properties ; Essential oils ; Solvent ; Terpene ; Angiosperm ; Article ; Chemistry ; Fractionation ; Isolation and purification ; Chemical Fractionation ; Chromatography, Gas ; Lamiaceae ; Monoterpenes ; Multivariate analysis ; Reproducibility of Results ; Solvents ; Thymol
  8. Source: Talanta ; Volume 79, Issue 3 , 2009 , Pages 695-699 ; 00399140 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0039914009003592