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Quantitative proteomic analysis of cellular responses to a designed amino acid feed in a monoclonal antibody producing Chinese hamster ovary cell line

Torkashvand, F ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.29252/.22.6.385
  3. Publisher: Pasteur Institute of Iran , 2018
  4. Abstract:
  5. Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing CHO cell line culture medium were investigated. Results: The glutathione synthesis, the negative regulation of the programmed cell death, proteasomal catabolic process, and the endosomal transport pathway were up-regulated in the group fed with a designed amino acid feed compared to the control group. Conclusion: Our findings could be helpful to identify new targets for metabolic engineering. © 2018, Pasteur Institute of Iran. All rights reserved
  6. Keywords:
  7. CHO cells ; Glutathione ; Monoclonal antibodies ; Proteomics
  8. Source: Iranian Biomedical Journal ; Volume 22, Issue 6 , 2018 , Pages 385-393 ; 1028852X (ISSN)
  9. URL: https://ibj.pasteur.ac.ir/article-1-2384-en.pdf