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Dual-sensitive hydrogel nanoparticles based on conjugated thermoresponsive copolymers and protein filaments for triggerable drug delivery

Ghaffari, R ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1021/acsami.8b01154
  3. Publisher: American Chemical Society , 2018
  4. Abstract:
  5. In this study, novel hydrogel nanoparticles with dual triggerable release properties based on fibrous structural proteins (keratin) and thermoresponsive copolymers (Pluronic) are introduced. Nanoparticles were used for curcumin delivery as effective and safe anticancer agents, the hydrophobicity of which has limited their clinical applications. A drug was loaded into hydrogel nanoparticles by a single-step nanoprecipitation method. The drug-loaded nanoparticles had an average diameter of 165 and 66 nm at 25 and 37 °C, respectively. It was shown that the drug loading efficiency could be enhanced through crosslinking of the disulfide bonds in keratin. Crosslinking provided a targeted release profile under reductive conditions using an in vivo agent, glutathione (GSH), or in the presence of trypsin. Cytocompatibility assay using HeLa and L929 fibroblast cells exhibited no adverse effect of nanoparticles on cell viability up to 1 mg/mL. Besides, the green fluorescence of curcumin confirmed the uptake of drug-loaded nanoparticles by cancer cells. The redox and temperature-sensitive nanoparticles are potentially useable for the efficient delivery of hydrophobic drugs to targeted regions having a triggerable release profile. Copyright © 2018 American Chemical Society
  6. Keywords:
  7. Drug delivery ; Keratin biopolymer ; Pluronic conjugates ; Stimuli-responsive copolymer ; Cell culture ; Controlled drug delivery ; Covalent bonds ; Hydrogels ; Hydrophobicity ; Lanthanum compounds ; Nanoparticles ; Proteins ; Sulfur compounds ; Clinical application ; Drug loaded nanoparticle ; Drug-loading efficiency ; Hydrogel nanoparticles ; Nanoprecipitation method ; Structural proteins ; Temperature-sensitive nanoparticles ; Thermo-responsive copolymers ; Targeted drug delivery
  8. Source: ACS Applied Materials and Interfaces ; Volume 10, Issue 23 , 17 May , 2018 , Pages 19336-19346 ; 19448244 (ISSN)
  9. URL: https://pubs.acs.org/doi/10.1021/acsami.8b01154