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Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns

Zandieh, M ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ab.2018.02.023
  3. Publisher: Academic Press Inc , 2018
  4. Abstract:
  5. This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes that LSPR biosensing can be considered as a sensitive, simple, and label-free method for endotoxin detection in the quality control laboratories. © 2018 Elsevier Inc
  6. Keywords:
  7. Endotoxin ; GLAD method ; Label-free ; LSPR biosensor ; Silver nanocolumns ; Silver nanoparticle ; Comparative study ; Endotoxin detection ; Escherichia coli ; Limit of detection ; Priority journal ; Refraction index ; Scanning electron microscopy ; Surface property ; Ultraviolet spectroscopy
  8. Source: Analytical Biochemistry ; Volume 548 , 2018 , Pages 96-101 ; 00032697 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0003269718301933