Loading...

Butanol Production from Bagasse Sugarcane Black Liquor by Native Strain and Clostridium Acetobutylicum Species

Hassani Atashgah, Milad | 2019

848 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51622 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Shayegan, Jalaleddin; Vossoughi, Manouchehr; Sedighi, Mahsa
  7. Abstract:
  8. Considering the limitations of fossil fuels in recent years, as well as increasing demand for biofuels, it is anticipated that future attention to biofuels will increase further. Among biofuels, due to some disadvantages of bioethanol and biodiesel fuel, there is a tendency to use biobutanol, which does not have the disadvantages of the mentioned fuels. The cost of chemical production of butanol also led researchers to use biotechnology production of butanol. According to studies, butanol production can be achieved by fermenting some sugars such as glucose, xylose, and ... by anaerobic microorganism, but the cost of using these sugars has resulted in the use of natural sources of these sugars instead of pure one. The economicaly of the process depends on the low value of the raw material (eg agricultural waste or paper waste). In this research, paper wastewaters by sugarence bagasse, which were produced as a source of sugar (carbon source) due to the limited resources of the wood, were used as well as in-waste strains for their fermentation. In the first step, black liquor is hydrolyzed with sodium bicarbonate and hot sulfuric acid to recover the desired sugar (xylose) to consume the strains from the sewage. Then, using active carbon dioxide, it was shown that most of the active carbon-containing inhibitors can be eliminated, and this deleterious effect on the growth of the strains. Subsequently, by finding insoluble strains for producing butanol through its growth in a culture medium containing xylose extracted from black liquor, only two strains susceptible to the production of butanol were isolated and purified, the best of which were the ability to produce biobotanol under the specified conditions, After DNA and PCR extraction, sequencing and identification were sequenced by determining the similarity of the 16S rRNA gene sequence, which was eventually found to be the strain of Clusteridium Acetobutylicum and is similar to the ATCC collection. A strain with the maximum production of butanol was performed by Box-Behnken based RSM method, which was at a concentration of 32 g /l of xylose, 1.96 g/l of ammonium acetate and 105 rpm is the highest predicted for butanol 3.69 g/l, which in real case, the highest amount achieved in the design of the experiment was 3.63 g/l, and also the efficiency and efficiency values of this process were also in the order of 0.16 g/g and 0.022 g/l.h. By comparing the butanol production of this strain with butanol production with Clostridium Acetobutylicum strain, it was observed that non-wood lignocellulosic waste containing xylose sugars was lower than other sources of butanol but its cost was low due to its cost It is very affordable. Also, the target strain recovered from black liquor can partly meet the needs of low-level butanol production
  9. Keywords:
  10. Biobutanol ; Block Liqour ; Response Surface Method ; Clostridium Osteoblutinum ; Xylose ; Biofuel ; Sugarcane Bagasse

 Digital Object List

 Bookmark

No TOC