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Producing of Peroxidase Enzymes using Pahnerochaete Chrysosporium for Decolorization of Molasses Wastewater

Alizadeh, Manijheh | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51818 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran
  7. Abstract:
  8. The treatment of water effluent from alcohol industries is among the most challenging industrial wastewater treatments. The challenge mainly arises from the high capacity of organic compounds allowing the growth of microorganisms and pollutants. Brown color of the wastewater also contributes to this phenomenon; it absorbs more sunlight which then reduces the oxygenation of water and thus adversely affecting the aquatic life. Moreover, the acidity of the wastewater decreases the pH level of agricultural soils, so its quality Thus, distillery wastewater, without any treatment can pose a serious threat to the environment. In our study, we investigate the decolorization of molasses wastewater using phanerochaete chrysosporium. These white rot fungi produce oxidative, extracellular enzymes such as manganese peroxidase, lignin peroxidase, and laccase, all of which playing important role in decolorization of various wastewaters.In this study, we reached 55-60% decolorization of molasses wastewater in different conditions including changes on agents in culture, temperature, and agitation speed. Experiments show the decolorization is more rapid in the shaken culture than in the stationary ones. Observing the activity of enzymes for several days, we record the maximum activity among enzymes every fourth day, 24 U/ml for manganese peroxidase, and 16 U/ml for lignin peroxidase. Laccase does not demonstrate any activity and manganese peroxidase and lignin peroxidase were main factors in decolorization. Empirical results show that both the optimum percentage of spores and amount of molasses wastewater is 10%. Moreover, results obtained by Design-Expert Software for the optimum amount of molasses and growth temperature are match to our experimental results (temperature 38℃ and about 10% molasses wastewater). Thiamin and veratryl alcohol had an effect on the activity of enzymes and the optimum amount of veratryl alcohol was 2mM. The activity of manganese peroxidase enzyme improves by adding Tween80 0.1% which then increases the decolorization. The enzyme was fixed on polyethylene packings, in order to be utilized several times without any change in its decolorization; however, decolorization decreased to 28% in the second batch and 17% in the third batch
  9. Keywords:
  10. Peroxidase Enzyme ; Molasses ; Phanerochaete Chrysosporium ; Manganese Peroxidase ; Laccas Enzyme ; Lignin ; Colored Textile Wastewater

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