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Separation and Microfluidic Analysis of one Cancer Biomarker (Exosome) by Means of Microfluidic Chip Microfluidc Analysis Exosome Toward Liquid Biopsy for Cancer Tumor Cell

Madani, Mohammad | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51815 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saidi, Mohammad Said; Firouzabadi, Bahar
  7. Abstract:
  8. The recent breakthroughs in the lab-on-a-chip technology will not be directly used as clinical devices unless the specific applications are carefully tested and certified. As there is a growing trend towards microfluidic devices for biomedical research, an active microfluidic device was developed. Common microfabrication methods were used to develop a microchip to separate and concentrate exosome and particular cell based on the dielectrophoresis phenomenon. Unique capabilities of the dielectrophoresis phenomenon make it a powerful tool for micro particle manipulation in micro scale. In this research, geometry for the mentioned purpose is designed and optimized. Three-dimensional microelectrodes are embedded in the microchannel to make the dielectrophoretic force. The mentioned force, near the edge of the microelectrodes, acts like a selective barrier to the exosomes. In this work, after reviewing the previously developed devices, theoretical backgrounds of the required phenomena were studied comprehensively. Next, numerical simulations were employed to compute the dielectrophoresis force, particle trajectories and the effective working parameters by means of the COMSOL Multiphysics® software. Three different fabrication methods were examined prior to the final device fabrication. In this experimental study, exosomes, nano particles and particular cell were introduced to the microchip and the efficiency of the separation was measured using optical microscopy. Moreover, the performance of the presented design was demonstrated using Polystyrene micro particles. A good agreement existed between the simulations and the experimental results, rendering a high quality performance for the microchip
  9. Keywords:
  10. Microfluidic System ; Separation ; Dielectrophoresis Technique ; Biomedical Microelectromechanical System (Bio-MEMS) ; Lab-on-a-Chip ; Microfabrication

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