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Design, Simulation and Fabrication of Integrated Centrifugal Microfluidic Platform for Separation and Lysis of Circulating Tumor Cells

Momeni, Maede | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52006 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Firoozbakhsh, Keykhosrow
  7. Abstract:
  8. Cancer diagnosis area has recently been in the limelight of the medical research and there exist an unremitting focus on the devices & technologies which enable cancer detection in its victims. Lately a genius diagnostic method based on isolation and entrapment of circulating tumor cells has been developed which pave the path for cancer identification. These circulating cells which are detached from the primary tumor are carried out through body by means of circulation system. They play key role in phenomenon called metastasis. Separating these rare cells from multifarious background blood cells, assessing their quantity can supply valuable information on the stage of disease as well as its lethality. By studying CTCs’ DNA content, genetic information on primary tumor can be assessed as well.Here we report the design & fabrication of microfluidic channels on polymeric disk. One of them utilizes the centrifugal force for cell isolation while the other (comprised of two functional unit for separation and mixing) is devised for lysis of the cell membrane.The separation unit exploits both active and passive methods. In other words, in addition to introducing novel geometry for channel, an external magnetic field is also employed to separate the target cells from the background cells. To make this external field functioning first the CTCs must be labeled with antibody conjugated nanoparticles.For optimal efficiency and better cell lysis, sharp edge obstacles are also embedded in mixer to enhance cell membrane destruction.
  9. Keywords:
  10. Centrifugal Microfluidics ; Circulating Tumor Cells (CTC) ; Separation ; Cell Lysis Membrane ; Magnetic Nanodot ; DNA Molecule ; Antibody

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