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Design and Fabrication of a Microfluidic Kidney Nephron-on-Chip Platform

Yahyazadeh Shourabi, Arash | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52469 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saeedi, Mohammad Saeed; Hajilouy Benisi, Ali; Moghadas, Hajar
  7. Abstract:
  8. In this manuscript, we designed and fabricated a novel integrated microfluidic Kidney Nephron-On-Chip. This chip is able to culture cell monolayers under various fluid shear stresses and divert osmotic pressure gradients while imposing four different concentrations of an injected drug on cells. The multi-layer platform consisting of two bubble-trappers to eliminate all unwanted bubbles from the system, a concentration gradient generator to generate four different concentrations of the injected drug, and a membrane-based cell culture chamber caple of providing renal cells with their in-vivo condition. Using colorimetric techniques, the bubble trapper ability was quantified at flow rates up to μl/min. Also, utilizing LIF and colorimetric methods, we observed that the concentration gradient generator works as expected from the simulations up to the flow rate of μl/min. The required time for the generator to reach the stable state was only five minutes. Finally, we investigated the chip’s ability to keep cells alive for long-term drug screening tests. Here, we used trypan-blue essay and found that more than cells would be cultured in the chamber under the dynamic culturing condition for days. The cell viability rate found to be 95 percent. Owing to the design and quantifications, the chip is able to replace traditional, complex drug screening procedures in laboratories as a portable, small and easy-to-use device, which reduces the costs of drug screening projects remarkably and leads organ-on-chip systems toward industrialization ever than before
  9. Keywords:
  10. Kidney on Chip ; Microfluidic Chip ; Nephron ; Fluid Shear Sress (FSS) ; Integrated Microfluidic Chip ; Bubble Trapper ; Concentration Gradient Generator

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