Loading...

Design and Fabrication of 2D Multi-Electrode Array (MEA)Devices to Evaluate Functionality of Neural Cell Network and Cardiac Cells

Ahmadvand, Tala | 2019

570 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52518 (05)
  4. University: Sharif University of Technology
  5. Department: Electrical Engineering
  6. Advisor(s): Fardmanesh, Mehdi
  7. Abstract:
  8. Microelectrode array technology is a broad platform for studying and characterization of the electrophysiological properties of excitable tissues derived from both brain and heart in vitro. Microelectrode arrays can either record or stimulate cells by accessing multiple sites of neural networks and cell tissues and detect signals from all sources around each electrode simultaneously. By using recorded signals, one can detect cell potential changes and its minor fluctuations. Unlike intracellular recording techniques, the noninvasive interface of microelectrode arrays with cells provides durable studying of neural networks and cell tissues. Using related microelectronics fabrication technologies, the impact of various materials including metal electrodes (gold, platinum, titanium nitride, etc.) and conductive polymers on different substrates have been investigated to achieve long-term recording of biological samples and a better understanding of cell characteristics and functions. This research focuses on the design and fabrication of two-dimensional microelectrode arrays using metallic electrodes on a silica substrate along with an electrical recording system. By precise adjustment of the size, distance, and number of the microelectrodes, one can achieve the high measurement selectivity and reliability that has been taken into account in the design of the microelectrodes. Gold/Titanium microelectrode arrays of optimized thicknesses, with 30-micron diameter and 60 electrodes have been designed and manufactured using sputtering and photolithography techniques. In addition, a capacitive coupling extracellular electrical recording system using microelectrode arrays, having 60-channels, with two-stage amplification, the gain of 1200 using multiplexers, with a noise level of less than 10 μV, the input bias current of 10 pA, and power consumption of 2 watts has been designed and fabricated. Finally, the performance of the microelectrode array and the electrical recording system have been evaluated at the Royan institute using live cells. In this evaluation, the noise level of the electrical recording system of 8 μV was obtained
  9. Keywords:
  10. Extracellular Recording ; Biocompatibility ; Neuron-Electrode Interface ; Neurons Signals ; Capacitive Coupling ; Microelectrode Array

 Digital Object List

 Bookmark

No TOC