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Modeling and Analysis of a Nano Particle Impinged on a Human Cell in Gene Therapy

Rostami, Majid | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52785 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Ahmadian, Mohammad Taghi; Firozbakhsh, Kikhosroo; Asghari, Mohsen
  7. Abstract:
  8. owning to the side effects and disadvantages of conventional methods of cancers treatments such as chemotherapy, currently, scientists are attempting to find new methods to replace them. Furthermore, many human diseases like SCID and Hemophilia are due to genetic disorders and scientists are also seeking to find permanent treatments instead of available temporary ones for them. in recent years, regarding the obtained achievement, Gene Therapy is being considered as a promising method for both cancers and genetic disorders treatment. But due to low efficiency of this method at this stage, there is an endevour among researchers for a more profound comprehension of the basics of gene therapy to ameliorate it. Gene Therapy process is done by various methods like Magnetofection, Sonoporation, hydrodynamics, Gene gun, Ultrasound, Needle injection and etc that each of them has its own pros and cons. but it seems that "Magnetofection" has more potential compared to other methods. therefore, this method was chosen for more study and investigation in the current research. then by reviewing the literature, it was determined that finding the required force for penetration and minimum impact initial velocity are of crucial importance in this method but are not well-studied yet. After studying basics of cell mechanics modeling and also the fundamentals of impact engineering and its relevant concepts of simulation by using FEM, the desired simulations were done using commercial softwares. the reported experimental value for the the required force for penetration of a 40 nm diameter nanotube is between 0.1 – 0.2 nN, while according to simulations results it is about 0.115 nN. this correlation shows that simulation results match well with experimental data. Also, the minimum required initial impact velocity by considering the mass of the nanotube was obtained to be 0.2458 m/s. regarding the great emphasis on the importance of Nanoparticle diameter-related effects on Magnetofection process, also a part was dedicated to this subject in the current research. repeating the done simulation of 40 nm diameter nanotube by four larger diameter values, resulted in that relationship between the required force for penetration and nanotube diameter follows a 2nd order polynomial; the larger the diameter, the more is the force as for diameters of 50,60,80 and 100 nm the required forces for penetration were respectively 0.178,0.260, 0.499 and 0.699 nN .by replacing the considered elastic material model for the previous simulation by hyperplastic ones, it was determined that the results of the elastic material model fit better with experimental data and the results considering hyperplastic material models are far from the reality. finally, by using a spherical nanoparticle with the same diameter instead of the nanotube, it was comprehended that the force required for penetration of a spherical nanoparticle is significantly less than the one of nanotube and this can be a beneficial point but regarding the electromagnetic-related matters, a nanotube might be more advantageous than a spherical nanoparticle
  9. Keywords:
  10. Cell Membrane ; Mechanical Faults ; Explicit Dynamic Analysis ; Magnetofection Method ; Gene Therapy ; Nanotube Penetration ; Cancer Treatment

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