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Experimental Investigation of Polyclorinated Biphenyls (PCBs)Bioremediation

Sharifi Dehsari, Hadiseh | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52821 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Ghobadi Nejad, Zahra
  7. Abstract:
  8. Polychlorinated biphenyls are a group of hydrocarbons that are composed of two benzene rings and chlorine admixture on it. Due to their special properties, these materials were added to oils for years to improve their properties. These compounds have many industrial applications in various industries, such as the production of fluorescent lamps, adhesives, dyes, and especially as a cooling and insulating liquid as the Askarel oil in power transformers. Due to the properties of these compounds, which include stability and bioaccumulation in fatty tissues, the wide use of these substances causes environmental contamination and leads to a variety of cancerous diseases. Among the cleaning methods, the use of biological methods, called bioremediation, is an economical, environmentally friendly and low-energy method than other methods. Isolation and purification of the indigenous microorganism from an infected environment as the most important tool for the application of the biochemical method in this study was investigated. In this way, indigenous microorganisms in seven polluted soil zones of Bandar Abbas plant were isolated by using Askarel as a Carbon source and also using two specific culture medium, and finally two microorganisms were selected for subsequent experiments. The detection of 16s rRNA on these two microorganisms demonstrated their nearness in terms of genetic sequencing with the strains of Lysinibacillus macrolides DSM54T and Bacillus firmus NBRC15306T. Regarding the possibility of degradation of polychlorinated biphenyl compounds with these microorganisms, various polychlorinated biphenyl compounds were degraded and analyzed by GC / MS tests. Also, using the Design expert software and the Response Surface Method (RSM), the optimal conditions for removing contaminants were determined by considering the factors include growth time of microorganism, microorganism concentration, temperature, pressure, and pollutants concentration
  9. Keywords:
  10. Polychlorinated Biphenyls ; Bioremediation ; Response Surface Method ; Lysinibacillus Macrolides ; Bacillus Firmus ; Askarel Oil ; Indigenous Microorganism Isolation

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