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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 53426 (08)
- University: Sharif University of Technology
- Department: Mechanical Engineering
- Advisor(s): Taghipoor, Mojtaba; Montazeri, Leila
- Abstract:
- In this work a new innovative microfluidic system for the selection of motile sperms was developed. The novel aspect of this work is based on the fact the motile sperms follow microchannel walls. Immotile sperms are not able to follow curved paths and therefore their movement toward the outlet gets hindered and most of them could not reach to the outlet. But motile sperms, moving alongside the microchannel walls, reach to the outlet and get separated from the immotile ones. After designing, 3 microfluidic chips having curved microchannels with curvature radius of 150, 300 and 600 micrometer and one with a straight microchannel were fabricated using photolithography and soft lithography. Being user friendly was the main feature of these chips so that accessories like syringe pump, centrifuge, power supply were not used and human semen sample was directly injected into the chips. Viability and motility tests results showed that sperms selected with the chips having curved microchannel with the curvature radius of 150 and 300 micrometers are better than those selected in the other chips. After this stage, the chip having the curved microchannel with the curvature radius of 150 micrometers was selected as the best chip and its output was compared with the outputs of conventional methods like sperm migration and density gradient centrifugation. This comparison showed that sperms selected with the chip are considerably better than those selected in the density gradient centrifugation regarding their motility. According to the features like simplicity, being user friendly, and low fabrication cost associated with the microfluidic chip and the fact that it performs better than the conventional method of density gradient centrifugation regarding the motility index of sperms selected by these two methods, the microfluidic chip, with minor amendments, has the potentiality to be used in clinical laboratories
- Keywords:
- Microfluidic Chip ; Infertility ; Separation ; Progressive Sperm ; Motile Sperm ; Curvature Radius
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