Design and Numerical Simulation of a Micro-chip for Separating CTCs from Peripheral Blood

Niknam, Hamid Reza | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 53946 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saeedi, Mohammad Saeed
  7. Abstract:
  8. According to studies, cancer is the second leading cause of death in the world and millions of people die from this disease every year. Early diagnosis is one of the most important factors in controlling and treating the disease. The ability of circulating tumor cells to diagnose and monitor the disease has led many researchers to focus on studying and researching these cells in recent years. However, due to the fact that the concentration of this type of cells in the blood is very low, their isolation is associated with challenges. Various methods isolate CTCs based on physical, biological and other differences in characteristics. In this study, we investigate performance of a microfluidic chip that separates particles using dielectrophoresis. First, a chip is introduced that has threedimensional electrodes that are isosceles triangle. Then all the effective parameters in the problem are discussed. Then the parameters that affect the problem and their optimal state are determined using the Taguchi algorithm. After performing the experiments, it was found that, the separation efficiency increases with decreasing channel width, channel height and flow rate. Also, the efficiency is directly related to the parameters of channel length and voltage difference. For the base angle of the electrode triangle, an angle of 30 ° was obtained as the optimal angle. Also, in the geometry, a sheath flow was used, which obtained the flow rate ratio optimal state is 3
  9. Keywords:
  10. Lab-on-a-Chip ; Microfluidic System ; Circulating Tumor Cells (CTC) ; Separation ; Dielectrophoresis Technique ; Cell Separation

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