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Investigating on Filtration of Solid Particles in Culture Media after Fermentation

Maleki, Maziar | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54160 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Roosta Azad, Reza
  7. Abstract:
  8. In biotechnology processes, in addition of culture media there is a solid phase which dispensed across the system.In most of bioprocesses, first operational unit for downstream treatment is the separation of this particles from fermentation liquor. In contrast with other categories like inorganic chemistry, solid particles in fermentation liquor (which the main part of it is biomass itself) have complicated physical & rheological properties. For example, all of biomass species are hydrophilic and sometimes form colloidal solutions.With this perspective, separation of solid phase from fermentation liquor is a hard challenge in this kind of processes.In this research, with regard of great extent of 2 phase solid-liquid environment in fermentation, we studying separation of solid phase and optimal approaches about it.At first, we analyze overall condition of the system and some of important laws and relations ruling it, with various experiments and repeating them in multiple passes we examine the clarity and enzyme activity in the filtrate. And depends on condition choosing the proper filter aid and also testing another type of filters. And finally define the critical point of preservation of enzyme activity of liquor.In past, some researches has been done about liquor clarification of some other enzymes although they’ve used other methodes than our’s. This method of protease enzyme liquor’s clarification has been done for the first time by filtration and help of soil and sand as filter aid.The result is fermented liquor turbidity has it’s absorbance droped from about 10 units to below 1 (in fact 90% of existing turbidity and impurement has been absorbed) while liquor’s enzyme activity has been kept up to almost 80%
  9. Keywords:
  10. Filtration ; Enzyme Activity ; Clarity ; Fermentation ; Biomass ; Filter Aid

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