Simulation, Evaluation and Fabrication of a Two-step Microfluidic System to Separate Circulating Tumor Cells

Mir Mohammad Sadeghi, Fatemeh Sadat | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54265 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosoughi, Manouchehr; Alamzadeh, Iran
  7. Abstract:
  8. Cancer is an abnormal growth of cells which is one of the major cause of worldwide’s mortality. Circulating Tumor Cells (CTCs) are rare cancer cells released from the primary or metastatic tumors and transported though the peripheral circulatory system and spreads in body and seize healthy organs. Early detection of CTCs can help in overtaking the cancer, hence isolation of CTCs is an essential step for many therapeutics. In spite of its clinical potential, the isolation and detection of CTCs has been a challenging task due to its rare presence amongst other blood cells (as low as 1–10 CTCs per billions of blood cells), similarity to white blood cell in size and also variability in terms of both morphological and biochemical markers. There are currently large number of methods for CTC separation which among them, microfluidic methods has shown promising results. In this thesis, an Inertial-DEP microchannel, is simulated by COMSOL Multiphysics and different design parameters are investigated. The inertial part, which is a spiral channel, functions to separate cells by size and after that the DEP channel separate cells by their dielectric characteristic. Comparing the Mixing Index (M.I.) parameter for various conformations an optimum design is obtained. With help of this simulated unit, isolation of CTCs takes place more efficiently. This 5TR1-mediated microchannel enhances the efficiency of the previous immunoaffinity method for isolation of CTCs
  9. Keywords:
  10. Cancer Cells ; Simulation ; Spiral Model ; Microfluidic System ; Separation ; COMSOL Software ; Dielectrophoresis Technique ; Circulating Tumor Cells (CTC)

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