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Synthesis of Magnetic Nanocomposite Scaffolds by Electrospinning Method and Study of Drug Release Behavior

Khodaei, Azin | 2021

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 54854 (48)
  4. University: Sharif University of Technology
  5. Department: Institute for Nanoscience and Nanotechnology
  6. Advisor(s): Bagheri, Reza; Madaah Hosseini, Hamid Reza
  7. Abstract:
  8. Controlled release is a crucial factor in tissue engineering and cancer-therapy applications. The main purpose of current research is to synthesis smart magnetic nanocarriers for hydrophobic drug and embedding them in a fibrous platform for anti-cancer/ tissue engineering applications. In this regard, three different drug delivery systems of magnetic nanocolloid, magnetic fibers and hydrogels were studied. In the first phase, superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized and then were modified using oleic acid and thermo-sensitive polymer of pluronic F127/F68. After characterization of this composite, Response Surface Methodology (RSM) was applied to model the lower critical solution temperature (LCST) of the polymer is shell and consequently the release temperature. In the next step, the nanoparticles with LCST of 47 ̊C were synthesized and curcumin as a hydrophobic drug was loaded in them. The hyperthermia efficacy and release behavior of curcumin as a function of temperature and pH were studied using these nanocarriers. Cellular studies on MG-63 osteosarcoma cell-line showed synergistic effect of temperature and curcumin at 41 ̊C as the optimum working temperature. In the second phase, a smart fibrous scaffold was designed to target tissue engineering/ cancer therapy purposes. In this regard, the developed magnetic nanocarrier was dispersed in silk fibroin (SF) solution and was casted on the surface of electrospun polycaprolactone (PCL) electrospun fibers. The mechanism and kinetics of curcumin release from this platform was studied and compared with the colloidal system. Cellular response exposing to these colloids was examined using MG-63 and hMSCs which proved specific toxicity on cancer cells at hyperthermia condition. As a result, healthy cells can survive to regenerate bone tissue. In the third phase of this project, the hierarchical hydrogels of SF/pluronic containing curcumin as an antibacterial agent were designed and synthesized to support skin tissue regeneration. The release behavior of curcumin was studied and cellular/bacterial tests proved the efficacy of hydrogel as an antibacterial biomaterial leading skin fibroblast cell. Overall, three different drug delivery systems with the purpose of cancer-therapy and regeneration of hard and soft tissue were introduced.
  9. Keywords:
  10. Drug Delivery ; Curcumin ; Pluronic ; Hyperthermia Magnetic Particles ; Smart Drug Delivery System ; Cancer Treatment ; Controlled Release ; Magnetic Carrier

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