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Design of Scaffolds with Multi-scale Engineered Microchannels

Mollajavadi, Mohammad Yasin | 2022

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 55378 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Saadatmand, Maryam
  7. Abstract:
  8. Building complex and functional tissues and organs is very challenging. One of the challenges is building an efficient network of blood vessels that can be used to facilitate the transport of nutrients and oxygen to the host. In addition to using channels for oxygen supply, another solution is to use oxygen-carrying materials. In this study, in addition to designing and simulating scaffolds with multi-scale microchannels, calcium peroxide was used to release oxygen and eliminate hypoxia in the scaffold. Here alginate is used as the main material for scaffolding. In an attempt to build a scaffold using a bio-printer, pluronic acid was also used as a sacrificial material to create canals. After printing and cooling the scaffold, liquid pluronic acid is removed from the scaffold to create channels. The simulation was performed with the help of Comsol software and the simulation results show the scaffold with the number of epsilon 0.2 (a parameter related to the distance between the channels) and the inlet channel width of 1.5 mm have a very good oxygen distribution at times without any hypoxic points and consequently proper cell growth occurs in the scaffold. Also, the results of tests such as oxygen release test, degradability, swelling, porosity and SEM images performed on the bulk of the material show that with increasing the percentage of calcium peroxide in the scaffold, oxygen release increased, although some properties such as biodegradability may be accelerated. The Alg100-CPO5 and Alg100-CPO10 samples did not show a significant difference in the rate of oxygen release, so that both samples could maintain an oxygen concentration in the solution of more than 5 mg/L for up to a week. With increasing CPO concentration, the porosity increased from about 50% in the control sample to about 80% in the Alg100-CPO5 sample, in fact, it increased to 60%. Increased scaffold porosity is of great importance for cell growth, proliferation and migration. Also, the maximum tensile strength in the two samples Alg100-CPO1 and Alg100-CPO5 is 63.22 ± 1.99 and 61.02 ± 2.57, respectively. The Alg100-CPO5 sample, in which the ratio of calcium peroxide to alginate is 5%, has been selected as a suitable sample for further studies.
  9. Keywords:
  10. Pluronic ; Alginate ; Hypoxia ; Calcium Peroxide ; Bioprint ; Multi-Scale Microchannels ; Bio-Printing ; Biodegradable Scaffold

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