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Optimization of Mucor miehei rennin production and recovery

Kazemi Vaysari, A ; Sharif University of Technology | 2002

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  1. Type of Document: Article
  2. Publisher: Sharif University of Technology , 2002
  3. Abstract:
  4. In this paper, fungal rennin production has been carried out by solid state fermentation of Mucor miehei NRRL 3420. The optimum dilution ratio, temperature and fermentation time of the enzymatic solution were determined to be 1:30, 40°C and 4 days after the beginning of the fermentation process, respectively. Also, the enzyme recovery by precipitation was studied using (NH4)2SO4, acetone and ethanol, respectively. The effects of pH, salt and solvent concentration on rennin recovery were studied and the optimum pH value as well as the optimum salt concentration were determined. The maximum specific activity of the enzyme was obtained by precipitation at pH of 6.5 and 50% saturated solution of (NH4)2SO4. When organic solvents were used for rennin recovery, the maximum specific activity of the enzyme was observed for acetone solution at pH of 6.5 and 65% saturation which is 17% less than the optimum specific activity obtained by 50% saturated solution of (NH4)2SO4. In comparison to ethanol, the use of acetone decreases the denaturation effect and the amount of the solvent needed for rennin recovery. The lower cost of the salt, higher stability of the recovered enzyme, more simple operation as well as more specific activity of the enzyme precipitated by (NH4)2SO4 in comparison to ethanol or acetone suggest salt precipitation as a reliable method for rennin purification
  5. Keywords:
  6. Dilution ; Temperature ; Fungus ; Fermentation ; Enzyme
  7. Source: Scientia Iranica ; Volume 9, Issue 1 , 2002 , Pages 99-104 ; 10263098 (ISSN)
  8. URL: http://scientiairanica.sharif.edu/article_2731.html