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An efficient strategy to overproduce glutamic acid in Corynebacterium glutamicum fermentation

Zamani, J ; Sharif University of Technology | 2001

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  1. Type of Document: Article
  2. Publisher: Sharif University of Technology , 2001
  3. Abstract:
  4. In this paper, effect of penicillin addition in enhancing the secretion of glutamic acid by Corynebacterium glutamicum was studied. The proper time of adding penicillin to maximize glutamic acid production was found to drift at repeated trials. In contrast, maximum acid productivity was obtained when penicillin was injected at a proper biomass concentration of about 7.7 gram dry weight per lit (gdw/l). Moreover, rate of consumption of sugar and ammonia through the course of glutamic acid fermentation was monitored. In production phase, these two rates were correlated properly with a ratio of 3.2:1 which is comparable to the theoretical stoichiometric value of 5:1. Therefore, through feeding a mixture of molasses and ammonia at the above mentioned ratio, an online double control of both sugar and pH in a fed-batch process based on single pH measurements becomes feasible
  5. Keywords:
  7. Source: Scientia Iranica ; Volume 8, Issue 3 , 2001 , Pages 203-206 ; 10263098 (ISSN)
  8. URL: https://www.sid.ir/paper/289388/en