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Production of lactic acid from whey by immobilized cells

Kazemi, A ; Sharif University of Technology | 2001

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  1. Type of Document: Article
  2. Publisher: Sharif University of Technology , 2001
  3. Abstract:
  4. In this paper, the production of lactic acid from deproteinized whey, using immobilized Lactobacillus casei on wood chips is investigated. The immobilization process was considered on various supports like wood chips, brick particle and porous glass by adsorption and on eggshell with glutaraldehyde by covalent bonding. Wood chips demonstrated the highest adsorption among all the supports and, therefore, was chosen as the best support for production. Batch production was studied at four temperatures and three different pH after five days. The highest concentration of lactic acid (16 g/1) was observed at T = 28°C and pH = 5.5. The optimum condition of temperature and pH for the continuous system was chosen as 32°C and 5, respectively, based on the results obtained in the batch system. The continuous reactor was designed as a packed-bed of L.casei immobilized on wood chips. The highest concentration of lactic acid produced in this system (14.8 g/1) was obtained with D = 0.2 h-1 after five days
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  6. Source: Scientia Iranica ; Volume 8, Issue 3 , 2001 , Pages 218-222 ; 10263098 (ISSN)
  7. URL: http://scientiairanica.sharif.edu/article_2753.html