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A novel formulation of simvastatin nanoemulsion gel for infected wound therapy: In vitro and in vivo assessment

Amoozegar, H ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jddst.2022.103369
  3. Publisher: Editions de Sante , 2022
  4. Abstract:
  5. Simvastatin, a well-known antihyperlipidemic drug, has antibacterial activity against a broad range of bacteria, especially Staphylococcus aureus. In present study, a nanoemulsion gel-based formulation containing Simvastatin was developed for infected wound therapy. Therefore, different formulations of Simvastatin nanoemulsion were prepared. Based on droplet size, polydispersity index and zeta potential, the best nanoemulsion formulation containing Simvastatin was selected for development of nanoemulsion gel formulation of drug using carbomer 934 as gelling agent. Thermodynamic stability of Simvastatin nanoemulsion was assessed at different conditions. The in vitro antibacterial activity against S. aureus as well as in vivo wound healing efficacy of both Simvastatin nanoemulsion and its nanoemulsion gel formulation were assessed. The droplet size, PDI, and zeta potential of selected Simvastatin nanoemulsion formulation were 75 nm, 0.3, and −29.4, respectively. The physical characteristics of nanoemulsion such as droplet size, charge and PDI did not change significantly in nanoemulsion gel formulation with viscosity of 11.12 Pas. Selected formulations of nanoemulsion and nanoemulsion gel of simvastatin were stable at 4 and 25 °C for 72 days and Minimum Inhibitory Concentration values of both nanoemulsion and nanoemulsion gel of drug against pathogenic strains S. aureus was 15.52 μg/ml which is two-fold lower than drug solution (31.25 μg/ml). Histological findings in mouse animal model of wound healing demonstrated the superiority of nanoemulsion gel belong other formulations. © 2022 Elsevier B.V
  6. Keywords:
  7. Antibacterial activity ; Nanoemulsion ; Simvastatin ; Wound healing ; Carbopol 934 ; Nanomaterial ; Oleic acid ; Polysorbate 80 ; Protein disulfide isomerase ; Animal experiment ; Animal model ; Animal tissue ; Bacterial growth ; Bacterial strain ; Comparative study ; Controlled study ; Dispersity ; Drug efficacy ; Drug formulation ; Drug solution ; Epithelization ; Fourier transform infrared spectroscopy ; Histology ; In vitro study ; In vivo study ; Male ; Minimum inhibitory concentration ; Mouse ; Nonhuman ; Particle size ; Phase separation ; Physical appearance ; Shear stress ; Staphylococcus aureus ; Surface property ; Thermodynamics ; Transmission electron microscopy ; Viscosity ; Wound contraction ; Wound infection ; Zeta potential
  8. Source: Journal of Drug Delivery Science and Technology ; Volume 72 , 2022 ; 17732247 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S1773224722002799