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Design and Fabrication af a Bioceramic-Polymer Composite Scaffold Using Bioprinter for Regeneration of Osteochondral Tissue

Hadian, Hamid Reza | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56100 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh
  7. Abstract:
  8. In this work, we have used a 3D printer and unidirectional ice-templating technique in conjunction, to fabricate a novel composite scaffold to facilitate local osteochondral defects tissue regeneration with a cell-free approach. Using ice-templating to induce radially-aligned porosity formation in type I bovine collagen is known to expedite host cells' migration into the scaffold which their ECM secretion shifts cellular milieu toward that of its neighboring tissue layer. We used numerical analysis to design and optimize appropriate freeze-casting mold, finding the optimum value for pin height and metal slab depth to be 4mm and 14mm, respectively. Collagen content equivalent to 2%w/v was found to be optimized with an average pore diameter and porosity value of 114.3µm and 88.1% respectively. We have incorporated Bioactive Glass-ceramic content into the radially aligned scaffold. moreover, a novelty designed mechanical support fabricated to improve mechanical properties of the collagen scaffold 50-folds up to 4.91 Mpa. Surficial BG incorporation as imaged by SEM help to seed hydroxyapatite formation and osteoblast cell proliferation. Biodegradation for composite scaffold showed 87% weight retainment after 6weeks incubation in PBS. In general, we have designed and successfully built a tri-part cell-free scaffold consisting of a cellular cue, a mechanical support with radial architecture, and a collagenous substance with radially aligned pores to induce cellular migration
  9. Keywords:
  10. Bio-Printing ; Bioactive Glass ; Unidirectional Freeze Drying ; Composite Scaffold ; Ice Templating Method ; Osteochondral Tissue Engineering ; Aligned Porosity ; Radially Porosity ; Three Dimentional Bioprinting

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