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Investigation of The Effect Of Microgravity On Bone Marrow Stromal Cell Growth And Infiltration On An Optimized 3d Structured Scaffold

Ahmadi, Armin | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 57007 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Vossoughi, Manouchehr; Haji Ebrahimi, Zahra; Janmaleki, Mohsen
  7. Abstract:
  8. To develop a novel cell culture method for enhancement of bone marrow stromal cell growth and infiltration into the 3d electrospun PCL-based scaffold, we utilized the RPM system to simulate microgravity. In current study, six different scaffolds are produced using the electrospinningelectrospraying technique. These scaffolds are compared based on their structural (average diameter and SEM micrographs), mechanical (Tensile strength, elongation and wettability) and biological (Biodegradability, bioactivity, biocompatibility, cell adhesion, cell infiltration and antibacterial quality) characteristics. It is noted that the porous nano-hydroxyapatite/titanium hydroxide/polycaprolactone (nHA/TiO2/PCL) scaffold with 89.3% possesses the highest porosity among the produced scaffolds. The tensile strength, elongation and contact angle values for this scaffold were 4.12 MPa, 163.4% and 89.1±0.3˚. XRD patterns before and after 21 days immersion in simulated body fluid (SBF) demonstrated high bioactivity for the selected scaffold. It was also noted that the selected scaffold had high cell infiltration and proliferation as well as antibacterial quality due to having TiO2. Scaffolds were taken into a 3 dimensional (3D) rotating bioreactor to simulate microgravity condition to improve the 3D cell growth and to achieve an optimal cell infiltration and proliferation. Moreover, during the culture of bone marrow stromal cells (BMSCs) on the selected scaffold, the cell adhesion and proliferation of BMSCs were presented well on both the surface and the cross-section of the scaffolds using Cryosection and MTT tests. All of these results strongly suggested that the nHA/TiO2/PCL scaffold to be promising in tissue engineering. This research suggests using international space station capabilities to culture the cells on this highly porous 3D scaffold
  9. Keywords:
  10. Microgravity ; Tissue Engineering ; Scaffold ; Electrospun Scaffold ; Cell Culture Medium ; Electrospray

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