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Development of printable nanoengineered composite hydrogels based on human amniotic membrane for wound healing application

Kafili, G ; Sharif University of Technology | 2023

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  1. Type of Document: Article
  2. DOI: 10.1007/s10853-023-08783-y
  3. Publisher: Springer , 2023
  4. Abstract:
  5. Recently, decellularized amniotic membranes (dAM) have attracted significant interest as a valuable source for the development of shear-thinning hydrogels and bioinks. However, the inferior rheological behavior and weak mechanical durability restrict the printability of the hydrogels and their stability after three-dimensional (3D) bioprinting. Therefore, a chemical or physical modification with biocompatible components is necessary to improve dAM-derived hydrogels’ properties. The present study proposes a strategy to fabricate printable dAM-derived hydrogels (DAMHs) supplemented with sodium alginate and Laponite nanoplatelets. Rheological experiments determined the key role of Laponite nanoplatelets in tailoring the shear-thinning behavior of the DAMHs. The dynamic mechanical modulus of the hydrogel was significantly enhanced (up to 16 folds, for example, storage modulus increased from ~ 0.5 to 8.4 kPa by adding 1% Laponite), which facilitated 3D printing of free-standing constructs without compromising biological properties. Meanwhile, excess agglomeration of the nanoplatelets in an ion-containing medium leading to nozzle clogging was observed at high Laponite concentrations (≥ 2%). Microstructural evaluations also revealed nanoplatelet-induced changes in the pore structures of the hydrogel, i.e., a finer pore structure was obtained. In vitro biological assays affirmed the biocompatibility of the nanoengineered hydrogels, while wound healing experiments revealed the positive effect of Laponite on fibroblast cell migration, as evidenced by ~ 30% enhancement in the wound healing rate after 36 h. Generally, the results obtained in this study demonstrate that the developed nanoengineered hydrogel provides suitable structural integrity and biocompatibility, highlighting its potential for therapeutic applications, particularly tissue engineering. Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature
  6. Keywords:
  7. Ecellularized amniotic membranes (dAM) ; Hydrogels ; Printable nanoengineered composite ; Three-dimensional (3D) bioprinting ; Human amniotic membrane ; Healing application
  8. Source: Journal of Materials Science ; Volume 58, Issue 30 , 2023 , Pages 12351-12372 ; 00222461 (ISSN)
  9. URL: https://link.springer.com/article/10.1007/s10853-023-08783-y?fromPaywallRec=true