Loading...

Architecting oxidized alginate methacrylate hydrogels with tunable characteristics by altering the sequence of the cross-linking steps, methacrylation reaction time, and polymer concentration

Jalali Kandeloos, A ; Sharif University of Technology | 2023

0 Viewed
  1. Type of Document: Article
  2. DOI: 10.1177/08853282231184294
  3. Publisher: SAGE Publications Ltd , 2023
  4. Abstract:
  5. In this study, biodegradable oxidized methacrylated alginate (OMA) hydrogels with controllable mechanical properties were engineered. An ionic and photo cross-linking combination was employed to fabricate dual cross-linked hydrogels. By altering the degree of methacrylation and polymer concentration, hydrogels with an elastic modulus of 4.85 ± 0.13 to 21.02 ± 0.91 kPa, controllable swelling, and degradation kinetics, and cross-link density in the range of 1.0 × 10−5 to 6.5 × 10−5 mol/cm3 were obtained. Moreover, evaluating the effect of cross-linking sequence on the hydrogels’ mechanical properties demonstrated that in comparison to the hydrogels fabricated by ionic cross-linking followed by photo-polymerization, hydrogels produced by photo-polymerization followed by ionic cross-linking retain a stiffer gel network with more compact structure. Cytocompatibility examination was performed via MTT assay against L929 fibroblasts, and all the hydrogel samples demonstrated high cell viability (>80%). The findings demonstrate the significant effect of the sequence of cross-linking as a novel tool to tune the OMA hydrogel’s final properties which can serve as a useful platform for tissue engineering applications. © The Author(s) 2023
  6. Keywords:
  7. Cross-linking sequence ; Hydrogels ; Ionic cross-linking ; Modified alginate ; Photo cross-linking
  8. Source: Journal of Biomaterials Applications ; Volume 38, Issue 1 , 2023 , Pages 25-38 ; 08853282 (ISSN)
  9. URL: https://journals.sagepub.com/doi/10.1177/08853282231184294